Potyviridae infects a wide variety of plants and those infections aresignificant constraint to agricultural productivity 1.
According toInternational Committee on Taxonomy of Viruses (ICTV), Potyviridae is the second largest plant virus family. This familyconsists of over 200 viruses divided into 8 genera. All of them are nakedviruses with helical symmetry and having positive sense single-stranded RNAgenome. 5′ end of their genome is linked with viral protein-genome linked (VPg)and 3′ end of the genome contains poly A tail 2. Macluravirus is a genus of Potyviridaewith the shortest genome (~8 kb) 3, 4.
According to ICTV, Macluravirus includes eight virus speciesnamely Alpinia mosaic virus (AlpMV), Artichoke latent virus (ArLV), Broad-leafed dock virus A (BDVA), Cardamom mosaic virus (CdMV), Chineseyam necrotic mosaic virus (CYNMV), Macluramosaic virus (MacMV), Narcissuslatent virus (NLV) and Yam chloroticmosaic virus (YCMV). Apart from these viruses, Large cardamom chirke virus (LCCV) 5 and a new yam infectingspecies (Accession number: KM201261) has been identified in India, and their coatprotein (CP) sequences are closely related with other macluraviruses. The genome of Macluraviruscodes a long open reading frame which is cleaved into nine proteins (HC-Pro,P3, 7K, CI, 9K, VPg, NIa, NIb and CP) and a small second ORF codes P3N-PIPO 6.The reported full-length genome sequence of Macluravirusspecies possess unique genome composition in Potyviridae, lacks complete P1 and N terminal region of HC-Pro codingregion 3, 4, 7, 8. Another interesting fact about macluraviruses, aretransmitted by aphids though it lacks the helper component region of HC-Pro, essentialfor aphid-mediated transmission 9. These uncovered properties of Macluravirus can be addressed by identificationof more new species and its genome analysis. But Macluravirus genus has full-length genome sequence for four virusesand any genus-specific conserved domain identification need an adequate numberof genome data. Wuhan poty-like virus 1is a vector-borne poty-like virus genome was identified during the screening ofinvertebrate RNA viruses and has been reported as unclassified RNA virus inNCBI 10.
In the present study, genome-wide Potyviridae motif searching, phylogenetic and recombinationanalysis have been done to confirm its association with Macluravirus.